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(A) Immunohistochemical staining of γH2AX, cGAS, STING, <t>IRF3,</t> and IFN-α was evaluated across multiple TMA slides and categorized as weak (1+), moderate (2+), or strong (3+). (B) Distribution of H-scores for each antibody across 164 cases. H-scores were categorized into two groups: Weak (0–150), Moderate to high (151–300). The Mann–Whitney U test was performed to compare two groups, *p < 0.001. Represents images acquired at 20 × magnification.
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(A) Immunohistochemical staining of γH2AX, cGAS, STING, <t>IRF3,</t> and IFN-α was evaluated across multiple TMA slides and categorized as weak (1+), moderate (2+), or strong (3+). (B) Distribution of H-scores for each antibody across 164 cases. H-scores were categorized into two groups: Weak (0–150), Moderate to high (151–300). The Mann–Whitney U test was performed to compare two groups, *p < 0.001. Represents images acquired at 20 × magnification.
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(A) Immunohistochemical staining of γH2AX, cGAS, STING, <t>IRF3,</t> and IFN-α was evaluated across multiple TMA slides and categorized as weak (1+), moderate (2+), or strong (3+). (B) Distribution of H-scores for each antibody across 164 cases. H-scores were categorized into two groups: Weak (0–150), Moderate to high (151–300). The Mann–Whitney U test was performed to compare two groups, *p < 0.001. Represents images acquired at 20 × magnification.
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(A) Immunohistochemical staining of γH2AX, cGAS, STING, <t>IRF3,</t> and IFN-α was evaluated across multiple TMA slides and categorized as weak (1+), moderate (2+), or strong (3+). (B) Distribution of H-scores for each antibody across 164 cases. H-scores were categorized into two groups: Weak (0–150), Moderate to high (151–300). The Mann–Whitney U test was performed to compare two groups, *p < 0.001. Represents images acquired at 20 × magnification.
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Image Search Results


(A) Immunohistochemical staining of γH2AX, cGAS, STING, IRF3, and IFN-α was evaluated across multiple TMA slides and categorized as weak (1+), moderate (2+), or strong (3+). (B) Distribution of H-scores for each antibody across 164 cases. H-scores were categorized into two groups: Weak (0–150), Moderate to high (151–300). The Mann–Whitney U test was performed to compare two groups, *p < 0.001. Represents images acquired at 20 × magnification.

Journal: PLOS One

Article Title: Prognostic value of cGAS-STING-IRF3 signaling in cholangiocarcinoma patients

doi: 10.1371/journal.pone.0342756

Figure Lengend Snippet: (A) Immunohistochemical staining of γH2AX, cGAS, STING, IRF3, and IFN-α was evaluated across multiple TMA slides and categorized as weak (1+), moderate (2+), or strong (3+). (B) Distribution of H-scores for each antibody across 164 cases. H-scores were categorized into two groups: Weak (0–150), Moderate to high (151–300). The Mann–Whitney U test was performed to compare two groups, *p < 0.001. Represents images acquired at 20 × magnification.

Article Snippet: Primary antibodies used included a rabbit polyclonal Gamma H2A.X antibody (phosphor S139) (γH2AFX; dilution 1:400, ab11174; Abcam), rabbit polyclonal anti-human c-GAS antibody (dilution 1:200, Proteintech, US), rabbit polyclonal anti-human STING antibody (dilution 1:3000, Proteintech, US), rabbit polyclonal anti-human IRF3 antibody (dilution 1:800, Proteintech, US), and rabbit polyclonal anti-human IFN alpha antibody (dilution 1:50, Proteintech, US).

Techniques: Immunohistochemical staining, Staining, MANN-WHITNEY

A . (A-C) FFPE from a CCA patients of H&E staining, 20X, scale bar = 100 µm of Well, Moderate and Poorly differentiated papillary subtype. (D-F) Weak (1+), Moderate (2+), or Strong (3+) of IRF3 expression patterns. Represents staining, 20X, scale bar = 100 µm. B . (A-C) FFPE from a CCA patients of H&E staining, 20X, scale bar = 100 µm of Well, Moderate and Poorly differentiated tubular subtype. (D-F) Weak (1+), Moderate (2+), or Strong (3+) of IRF3 expression patterns. Represents staining, 20X, scale bar = 100 µm.

Journal: PLOS One

Article Title: Prognostic value of cGAS-STING-IRF3 signaling in cholangiocarcinoma patients

doi: 10.1371/journal.pone.0342756

Figure Lengend Snippet: A . (A-C) FFPE from a CCA patients of H&E staining, 20X, scale bar = 100 µm of Well, Moderate and Poorly differentiated papillary subtype. (D-F) Weak (1+), Moderate (2+), or Strong (3+) of IRF3 expression patterns. Represents staining, 20X, scale bar = 100 µm. B . (A-C) FFPE from a CCA patients of H&E staining, 20X, scale bar = 100 µm of Well, Moderate and Poorly differentiated tubular subtype. (D-F) Weak (1+), Moderate (2+), or Strong (3+) of IRF3 expression patterns. Represents staining, 20X, scale bar = 100 µm.

Article Snippet: Primary antibodies used included a rabbit polyclonal Gamma H2A.X antibody (phosphor S139) (γH2AFX; dilution 1:400, ab11174; Abcam), rabbit polyclonal anti-human c-GAS antibody (dilution 1:200, Proteintech, US), rabbit polyclonal anti-human STING antibody (dilution 1:3000, Proteintech, US), rabbit polyclonal anti-human IRF3 antibody (dilution 1:800, Proteintech, US), and rabbit polyclonal anti-human IFN alpha antibody (dilution 1:50, Proteintech, US).

Techniques: Staining, Expressing

(A–E) Kaplan–Meier survival curves comparing patients with low and moderate-to-high expression of γH2AX, cGAS, STING, IRF3, and IFN-α. P-values were calculated using the log-rank test.

Journal: PLOS One

Article Title: Prognostic value of cGAS-STING-IRF3 signaling in cholangiocarcinoma patients

doi: 10.1371/journal.pone.0342756

Figure Lengend Snippet: (A–E) Kaplan–Meier survival curves comparing patients with low and moderate-to-high expression of γH2AX, cGAS, STING, IRF3, and IFN-α. P-values were calculated using the log-rank test.

Article Snippet: Primary antibodies used included a rabbit polyclonal Gamma H2A.X antibody (phosphor S139) (γH2AFX; dilution 1:400, ab11174; Abcam), rabbit polyclonal anti-human c-GAS antibody (dilution 1:200, Proteintech, US), rabbit polyclonal anti-human STING antibody (dilution 1:3000, Proteintech, US), rabbit polyclonal anti-human IRF3 antibody (dilution 1:800, Proteintech, US), and rabbit polyclonal anti-human IFN alpha antibody (dilution 1:50, Proteintech, US).

Techniques: Expressing